$ 209.76
Details
Thermo Scientific™ RapidOut DNA Removal Kit rapidly and safely removes genomic DNA from total RNA and mRNA preparations. Complete digestion of DNA and safe removal of DNase I from the digestion reaction is ensured without RNA-damaging steps, such as heating or organic extraction. First, the RNA sample is treated with recombinant RNase-free DNase I to levels below the limit of detection by routine PCR. DNase I is safely removed subsequently using proprietary DNase Removal Reagent (DRR).DRR efficiently binds DNase I and the complex is collected at the bottom of the tube by centrifugation. The purified RNA is collected as a supernatant. The RNA after RapidOut procedure is free from DNA contamination and free of DNase I. It is ready to use in different applications including end-point or real-time RT-PCR, cloning, microarrays, and Northern blotting.Thermo Scientific RapidOut DNA Removal Kit rapidly and safely removes genomic DNA from total RNA and mRNA preparations. Complete digestion of DNA and safe removal of DNase I from the digestion reaction is ensured without RNA damaging steps, such as heating or organic extraction. First, the RNA sample is treated with recombinant RNase-free DNase I to levels below the limit of detection by routine PCR. DNase I is safely removed subsequently using proprietary DNase Removal Reagent (DRR). DRR efficiently binds DNase I and the complex is collected at the bottom of the tube by centrifugation. The purified RNA is collected as a supernatant. The RNA after RapidOut procedure is free from DNA contamination and free of DNase I. It is ready to use in different applications including end-point or real-time RT-PCR, cloning, microarrays, and Northern blotting. HighlightsEfficient - complete ds and ssDNA digestion and proprietary technology for DNase I removalRapid - single step sufficient for complete DNase I removalSafe - no need for toxic organic extractions or RNA-damaging heating steps Applications Main ApplicationsRNA isolation and RNA analysis, particularly RT-qPCR and RT-PCR (customers performing expression analysis of low transcription level genes.Customers performing ds-cDNA synthesis from total RNA preps. Other applicationsElimination of DNA from RNA for microinjections and transfection.Elimination of DNA from RNA prior microarray analysis.Elimination of DNA from RNA prior Northern blot analysHazardous:No> Quality Control:Ribonuclease Assay>For DRR:>> No contaminating RNase was detected after incubation of 10 μL of DRR with 160 ng of 2 kb RNA transcript for 4 hours at 37°C.>For DNase I:>> No contaminating RNase was detected after incubation of 5u of DNase I with 160 ng of 2kb RNA transcript for 4 hours at 37°C.>DNase I removal efficiency> RNA sample after RapidOut procedure was incubated with 1 μg of supercoiled plasmid DNA for 15 min at 37°C. Less than 20% of supercoil DNA was transformed to nicked and linear forms.>Functional testing>Of the kit:>> DRR is functionally tested in RT-qPCR amplification reaction with RNA treated with RapidOut DNA Removal Kit.>Of DNase I:>> DNase I is functionally tested in a unit activity assay: one unit of the enzyme completely degrades 1 μg of pUC19 DNA in 10 min at 37°C.> Storage Condition:-20°CMain ApplicationsRNA isolation and RNA analysis, particularly RT-qPCR and RT-PCR (customers performing expression analysis of low transcription level genesCustomers performing ds-cDNA synthesis from total RNA prepsOther applicationsElimination of DNA from RNA for microinjections and transfectionElimination of DNA from RNA prior microarray analysisElimination of DNA from RNA prior Northern blot analysi
