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The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.Purity: Based on immunoelectrophoresis at an antigen concentration of 20 mg/ml, the pattern of precipitation against goat anti-rat whole serum is the same as that against goat anti-rat IgG, Fc fragment specific. No precipitin line was detected against goat anti-rat IgA, alpha chain specific or goat anti-rat IgM,μ chain specific. This antibody is recommended for use in blocking assays, immunohistochemical, immunocytochemical and immunofluorescent applications.Storage: Store at 2-8ºC under sterile conditions. Dilute only enough antibody for a single day’s use.Country of Origin: USA
