RNase T1 (1000 U/µL), RNase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues, Each

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Thermo Scientific Rnase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues (see Figure 1 in Supporting Data). It cleaves the phosphodiester bond between the 3'-guanylic residue and the 5'-OH residue of adjacent nucleotides with the formation of corresponding intermediate 2', 3'-cyclic phosphate. The reaction products are 3'-GMP and oligonucleotides with a terminal 3'-GMP.Rnase T1 does not require metal ions for activity.ApplicationsRemoval of RNA from DNA preparationsRNA sequencingRibonuclease protection assays. Used in conjunction with RNase ARemoval of RNA from recombinant protein preparationsDetermination of the level of RNA transcripts synthesized in vitro from DNA templates containing a @@@G-less cassette@@@