RPMI 1640 Medium, HEPES, Originally developed to culture human leukemic cells in suspension and as monolayer, Case of 6

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Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. This RPMI is modified as follows: with L-glutamine, HEPES, phenol red. Contains reducing agent glutathione, high concentrations of vitamin biotin, vitamin B12, and PABAVitamins inositol and choline are present in high concentrationsContains no proteins, lipids, or growth factorsRequires supplementation, commonly with 10% Fetal Bovine Serum (FBS)Uses sodium bicarbonate buffer system (2.0g/L), and therefore requires 5-10% CO2 environment to maintain physiological pHSustainable packagingCell Culture, Mammalian Cell Culture