RPMI 1640 Medium, no glutamine, Originally developed to culture human leukemic cells in suspension and as a monolayer, Case of 10

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Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.RPMI is modified as follows: with phenol red; without HEPES, L-glutamineRPMI 1640 Medium is unique from other media because it contains reducing agent glutathione and high concentrations of vitaminsContains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle MediumVitamins inositol and choline are present in high concentrationsContains no proteins, lipids, or growth factorsRequires supplementation, commonly with 10% Fetal Bovine Serum (FBS)Uses sodium bicarbonate buffer system (2.0g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pHSustainable packagingCell Culture, Mammalian Cell Culture