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Specifications Synonyms: threo-1,4-Dimercapto-2,3-butanediol; Cleland's reagent; DTTFormula: HSCH2CH(OH)CH(OH)CH2SHFormula Weight: 154.25CAS No.: 3483-12-3Purity: ≥99.0% (RT)Storage Temperature: 2-8°CApplication: An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).Anion Traces: sulfate (SO42-): ≤50 mg/kgAssay: ≥99.0% (RT)Cation Traces: Mn: ≤5 mg/kgImpurities: ≤0.3% 4,5-dihydroxy-1,2-dithianeMP: 42-43°CSolubility: H2O: soluble50 mg/mL, clear, colorlessStorage Temp.: 2-8°C
