SP6 RNA Polymerase (20 U/µL), SP6 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for its double-stranded promoter, Each

Details

Thermo Scientific™ Bacteriophage SP6 RNA polymerase is DNA-dependent RNA polymerase with strict specificity for their respective double-stranded promoters.It catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter. Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides) Recommended for:Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing. Note:Consensus promoter sequences: AATTAACCCTCACTAAA G GGAGA - T3 TAATACGACTCACTATA G GGAGA - T7 ATTTAGGTGACACTATA G AAGNG - SP6 The position in bold ( 1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through 3 are critical for transcription, and they must be a G and a purine base, respectively (9).