T7 RNA Polymerase (20 U/µL), T7 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for its double-stranded promoter, Each

Details

Thermo Scientific™ Bacteriophage T7 RNA polymerase is DNA-dependent RNA polymerase with strict specificity its respective double-stranded promoter.It catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter. Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides) Recommended for:Synthesis of unlabeled and labeled RNA that can be used:for hybridization, in vitro RNA translation; as aRNA, siRNA, substrate in RNase protection assays, template for genomic DNA sequencing. Note:Consensus promoter sequences: TAATACGACTCACTATA G GGAGA. The position in bold ( 1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through 3 are critical for transcription, and they must be a G and a purine base, respectively.