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Specifications Synonym: 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, MTT, Methylthiazolyldiphenyl-tetrazolium bromideCAS Number: 298-93-1Empirical Formula (Hill Notation): C18H16BrN5SMolecular Weight: 414.32Beilstein Registry Number: 4081397EC Number: 206-069-5MDL Number: MFCD00011964PubChem Substance ID: 24896603Packaging 1, 5, 10 g in glass bottle; 100, 250, 500 mg in glass bottleApplication Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation.1,2,3,4,5 MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent6 and for the detection of NAD.7 ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used.8 MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.9Assay: 98%Form: powderMP: 195 °C (dec.)(lit.)Solubility: H2O: soluble5 mg/mL LStorage Temp.: 2-8°C
